CHANG Medium BMC
Complete medium for the culture of bone marrow and other hematopoietic cells for chromosomal analysis
Further Information
CHANG Medium BMC was developed for the primary culture of Clinical Human Bone Marrow cultures for use in karyotyping and other genetic testing using existing protocols based on RPMI Medium 1640, 10% GCT-CM and gentamicin.
Packaged in single bottles, ready-to-use with no mixing required. For in vitro diagnostic use.
Storage – Store below -10°C
| Size | SKU |
|---|---|
| 100mL | 91004-100mL |
| 500mL | 91004-500mL |
Brochures
White Papers & Research Articles
First allow the supplement to equilibrate to room temperature. Once equilibrated, under aseptic conditions the appropriate amount of sterile, distilled water can be added to the lyophilizate. Swirl the vial gently. It may be necessary to incubate the vial at 37°C for 5 to 10 minutes to completely dissolve the supplement. Aseptically transfer the entire contents of the reconstituted CHANG Medium C supplement to the bottle of CHANG Medium B basal. Mix the complete CHANG Medium C well by swirling the bottle. Supplement aseptically with sterile L-glutamine, 10.0 mL/L. Antibiotics may be added if desired.
All products manufactured at Irvine Scientic are subjected to rigorous Quality Control testing before they are released for sale. CHANG Medium products are tested for sterility, mycoplasma, pH, osmolality, and endotoxin. Functional testing is performed by an independent clinical testing lab, the Genetics Center in Orange, CA. Details on tests performed are available on the Certicate of Analysis (CoA) which accompanies each shipment.
When the media is more yellow in color it is an indicator that the pH has changed and is more acidic. In the Product Insert, we recommend placing the bottle in a 5% - 8% CO incubator with the cap slightly loosened for about 30 minutes to equilibrate the pH.
We recommend that all CHANG Medium that are stored frozen, below -10°C, should be limited to no more than two (2) freeze/thaw cycles to preserve the performance of the product. Please review each of the specic product inserts for storage recommendations.
Yes, all CHANG Medium formulations contain both FGFs and insulin.
Trypsin Solution 10X is a 2.5% solution of 1:250 trypsin. The 1:250 designation indicates the tryptic activity based on standardized testing. This ratio indicates that one part trypsin will digest 250 parts casein in the USP activity assay.
The main point of emphasis is to ensure that you use smaller asks (i.e. T-12.5, not T25 or larger) to seed the pellet containing cells with no more than 2.5 ml of media. By using this method, the cells will be concentrated allowing for quicker attachment to the substrate and allow for easier exchange of growth factors among the cells. Another critical point is to ensure adequate gas exchange to prevent the pH from lowering below what is recommended to
prevent the creation of an acidic environment.
